Analysis and sorting by flow andimage cytometry
Lecturers and Teaching Assistants
Dr. Ayala Sharp
Course Schedule and Location
When / Where:
5-9/11/2017; Flow Cytometry lab, Wolfson, room 21. ,
No. of hours (laboratories):
Field of Study, Course Type and Credit Points
Life Sciences: Laboratory; Elective; 0.50 points
Life Sciences (Systems Biology Track): 0.50 points
Life Sciences (Molecular and Cellular Neuroscience Track): 0.50 points
lectures: De Picciotto meeting room 25. Every day from 9-10.30.
Lab: Flow Cytometry lab, Wolfson, room 21.
The lab sessions are: morning-10.45-13.00, afternoon-13.46-16.00.
Language of Instruction
Attendance and participation
Pass / Fail
Grade Breakdown (in %)
Scheduled date 1
Date / due date
Estimated Weekly Independent Workload (in hours)
Principles of operation of Flow and Image Cytometers: the flow systems, sample excitation and illumination, sample detectors.
Applications of Flow and Image Cytometry: measurement of multiple color staining, DNA measurements, Nuclear - cytoplasm translocations.
Cell Sorting and analysis of yield and purity.
Preparation of samples.
Upon successful completion of this course students should be able to:
Discuss the principles and some applications of Flow and Image Cytometry.
Run samples on Flow and Image Cytometers.
Analyze samples with multi-color staining.
Sort multi-color cell populations to test tubes.
Perform cell cycle analysis.
Shapiro H. M - Practical Flow Cytometry.
Givan A. L - Flow Cytometry first principles.
Ormerod M. G. - Flow Cytometry
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