Lecturers and Teaching Assistants
Dr. Raya Eilam-Altstadter
Dr. Afroditi Tsitsou-Kampeli
Course Schedule and Location
The course will take place in 4 rounds: on August 16-17; 18-19; 23-24; 25-26. ,
Field of Study, Course Type and Credit Points
Life Sciences (Molecular and Cellular Neuroscience Track): Laboratory; Obligatory; Regular; 1.00 points
Life Sciences: Laboratory; Elective; Regular; 1.00 points
Life Sciences (Brain Sciences: Systems, Computational and Cognitive Neuroscience Track): Laboratory; Elective; Regular; 1.00 points
Location: Leonescu Building, Room number 101.
Starting each day at 9 am.
Attendance and participation
Estimated Weekly Independent Workload (in hours)
This course is a hands-on animal neuroanatomy laboratory will be given as a condense 2 days session (8 hours each day). The objectives of this lab course is to expose graduate students of the WIS who are interested in pursuing studies in Brain Sciences and Neuroscience to a variety of neurohistological methods. This course will include:
- Basic histological techniques: sectioning, various types of staining.
- Principles of microscopy.
- Analysis of stained histological material from various brain areas of mice: cerebellum, neocortex, spinal cord, neuromuscular junction.
- Comparison between brains of healthy animals versus various animal models of brain disease.
- During the course the students will prepare sections of brain tissue for visualization using a fluorescent microscope; the tissue must first be fixed, cut and stain to bring out cellular details that would otherwise be difficult to observe. Students will inspect and identify various brain regions and neural tracts in health and disease, mainly those characterizing the motor system (pyramidal and extrapyramidal trackts) .
- At the end of the lab the students will have to submit a report guided by leading questions. To this end, each pair will get individual guidance for macroscopic photography and data processing. Dates for this guidance will be scheduled with each pair individually
Upon successful completion of this course, students will be able to
- Prepare tissues for staining.
- Discuss tissue immunostaining.
- Identify brain regions, structures, and neural tracts.
- Distinguish between normal and pathological brain tissues.
- Process data using Adobe Photoshop and ImagePro Plus software.