Course Identification

Title:

Protein purification, crystallisation and structural analysis

Code:

20213322

Lecturers and Teaching Assistants

Lecturers:

Dr. Shira Albeck, Dr. Orly Dym, Dr. Yael Fridmann-Sirkis

TA's:

N/A

Course Schedule and Location

Year:

2021

Semester:

Second Semester

When / Where:

June 6 - June 10 2021 between 10:00 - 17;00, FGS, Lab

First Lecture:

06/06/2021

End date:

10/06/2021

No. of hours (laboratories):

20

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; Regular; 0.50 points
Chemical Sciences: Laboratory; Elective; Regular; 0.50 points
Life Sciences (Molecular and Cellular Neuroscience Track): Laboratory; Elective; Regular; 0.50 points
Life Sciences (Computational and Systems Biology Track): Laboratory; Elective; Regular; 0.50 points

Comments

N/A

Prerequisites

No

Restrictions

Participants:

20

Language of Instruction

English

Attendance and participation

Obligatory

Grade Type

Pass / Fail

Grade Breakdown (in %)

Attendance

100%

Evaluation Type

Final assignment

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

3

Syllabus

Bioinformatic analysis of proteins.
Protein purification using: affinity, ion exchange, size exclusion, hydrophobic interaction, AS precipitation, refolding IBs.
Protein analysis: removal of tags, SDS PAGE, dialysis, concentration of proteins and methods to determine protein concentration.
Protein evaluation of fold
Crystallization of proteins
Analysis of protein- protein interaction using MST and Biacore
3D structure determination and preliminary structure analysis.

Learning Outcomes

Upon successful completion of this course students should be able to:

Demonstrate basic knowledge and experience in protein bioinformatics, purification, analysis, crystallization, and 3D structure determination.

Reading List

N/A

Website

N/A