Course Identification

Title:

Analysis of proteins: Chromatography and mass spectrometry

Code:

20233312

Lecturers and Teaching Assistants

Lecturers:

Dr. Yishai Levin

TA's:

N/A

Course Schedule and Location

Year:

2023

Semester:

Second Semester

When / Where:

N/A

First Lecture:

19/06/2023

End date:

09/07/2023

No. of hours (laboratories):

28

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; Regular; 1.00 points
Chemical Sciences: Laboratory; Elective; Regular; 0.50 points

Comments

19.06.23 09:00-11:00 mass spec basics-Room 2, FGS
20.06.23 09:00-13:00 proteomics basics-Room 2, FGS
21.06.23 09:00-11:00 Native MS theoretical (changed from the 15th due to unexpected circumstances)-Room B, FGS
02.07.23-05.07.23 09:00-14:00 practical part in groups (Gili and Yishai)
09.07.23 09:00-11:00 exam (Gili and Yishai)

Prerequisites

No

Restrictions

Participants:

30

Language of Instruction

English

Registration by

Registration By:22/05/2023

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

Attendance

50%

Final:

50%

Evaluation Type

Seminar

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

1

Syllabus

Analysis of proteins provides unparalleled insight into the function of many biological systems. Chromatography and mass spectrometry are the core technologies that provide the capability to measure proteins in their native or identify and quantify thousands of proteins in a single experiment (termed bottom up proteomics).

The course will provide theoretical background of discovery proteomics and native protein analysis, including how raw data of typical experiments are produced, what information it provides, how to process the data and interpretation.

The course will also include live demonstrations at the de Botton Institute for Protein Profiling and at Prof. Michal Sharon’s laboratories of how to run samples on the instruments and process data.

Learning Outcomes

Upon successful completion of this course students should be able to:

Demonstrate understanding of the basics of chromatography and mass-spectrometry.
Demonstrate understanding of the basics of mass spectrometry including mass spectrometry for characterizing the structural properties of protein complexes.
Prepare samples for the different methods of analysis, appreciate data collection and analysis.
Discuss the various applications, limitations and restrictions of the various methods.

Reading List

N/A

Website

N/A