Course Identification

Protein purification, protein-protein interactions and crystallization & structural analysis
20233322

Lecturers and Teaching Assistants

Dr. Shira Albeck, Dr. Orly Dym, Dr. Yael Fridmann-Sirkis, Mr. Jossef Jacobovitch, Ms. Shelly Hamer-Rogotner
Hedva Hamawi

Course Schedule and Location

2023
Second Semester
11-20 of JUNE 2023., FGS, Lab
11/06/2023
20/06/2023
20

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; Regular; 1.00 points
Chemical Sciences: Laboratory; Elective; Regular; 1.00 points

Comments

11-20 of JUNE 2023.

Prerequisites

No

Restrictions

30

Language of Instruction

English

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

100%

Evaluation Type

Laboratory

Scheduled date 1

20/06/2023
N/A
-
N/A

Estimated Weekly Independent Workload (in hours)

3

Syllabus

 ”Protein crystallization, structure analysis, modeling and visualization by PyMOL”

·       Bioinformatic tools for protein analysis.

·       Protein crystallization – techniques, strategies and tips.

·       Correlation between proteins sequence-function-structure.

·       Protein structure prediction.

·       Protein visualization – PyMol

“Protein purification, characterization & protein-protein interaction analysis”

  • Protein purification using a variety of tags & biophysical properties
  • Handling purified proteins:

Concentrating proteins

Buffer exchange

Labeling

Determining protein concentration

Protein stabilization

  • Analysis of protein:

Oligomeric state by SEC

Stability by DSF & DLS

Analysis of protein-protein interaction:  ITC, MST & Biacore

 

 

Lab course schedule: Protein purification & Analysis (11-20 June)

 

Sunday (11.6)

9:00 – 16:00    Protein purification lectures + experiments @ FGS

 

Monday (12.6)

9:00 – 16:00    Protein purification lectures + experiments @ FGS

 

Tuesday (13.6)

9:00 - 14:00     Protein analysis @ Levin lab

14:00 - 16:00   Protein purification lecture + experiment @ FGS

 

Wednesday (14.6)

9:00 - 10:30     Protein purification lab @ SPU (Benozyo #118)

                       

10:30-13:00     Protein purification exersize @ Benoziyo Biochemistry seminar room

 

13:00 - 16:00   Protein analysis lecture + experiment @ Levin lab

 

Thursday (15.6)

 

9:00 - 13:30     Protein purification lecture + experiment @ FGS

13:30 - 16:00   Protein analysis experiment @ Levin

 

Sunday (18.6)

 

9:00 – 16:00 Protein crystallization lectures & experiment @ FGS

 

Monday (19.6)

 

9:00 – 16:00 Protein crystallization lectures & experiment @ FGS

 

Tuesday (20.6)

 

9:00 – 13:00 Protein fold anaylsis Pymol @ Benozyo Biochemistry seminar room

 

13:00 – 16:00 Exam @ Benozyo Biochemistry seminar room

Learning Outcomes

Upon successful completion of this course students should be able to:

  • Demonstrate basic knowledge and experience in protein bioinformatics, protein purification, analysis of protein-protein interactions, crystallization of proteins and 3D structure analysis.

Reading List

N/A

Website

N/A