Course Identification

The RNA world
20243471

Lecturers and Teaching Assistants

Prof. Rotem Sorek, Prof. Eran Hornstein, Prof. Igor Ulitsky
Dr. Iddo Magen

Course Schedule and Location

2024
First Semester
Tuesday, 11:15 - 13:00, Belfer, Botnar Auditorium
12/12/2023
27/02/2024

Field of Study, Course Type and Credit Points

Life Sciences: Lecture; Elective; Core; 2.50 points
Life Sciences (Molecular and Cellular Neuroscience Track): Lecture; Elective; Core; 2.50 points
Life Sciences (Computational and Systems Biology Track): Lecture; Elective; Core; 2.50 points
Life Sciences (ExCLS Track): Lecture; Elective; 2.50 points
Mathematics and Computer Science (Systems Biology / Bioinformatics): Lecture; Elective; 2.50 points

Comments

This course will be held by hybrid learning

Prerequisites

Student should hold an undergraduate degree in Life Sciences

Restrictions

120

Language of Instruction

English

Attendance and participation

Expected and Recommended

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

100%

Evaluation Type

Examination

Scheduled date 1

N/A
N/A
-
N/A

Scheduled date 2

N/A
N/A
-
N/A

Estimated Weekly Independent Workload (in hours)

N/A

Syllabus

RNA emerges in recent years as a dominant mechanism for regulation of biological processes at all levels. The aim of this course is to explore advanced topics in RNA-based regulation. The course will discuss mechanisms such as microRNA, alternative splicing, RNA editing and nonsense mediated RNA decay. A strong focus will be put on microRNA biochemistry, target selection and developmental impact. We will also discuss small RNA based regulation in prokaryotes including riboswitches, sRNA, and CRISPR.

  1. Alternative splicing
  2. Alternative splicing regulation
  3. Nonsense mediated RNA decay
  4. RNA editing
  5. microRNA - introduction
  6. Posttranscriptional processing of microRNA
  7. microRNA:target pairing and RISC function
  8. miRNA target genomics
  9. microRNA function in vertebrate development
  10. On miRNA in plants
  11. OncomiRs and tumor suppressor miRNAs
  12. lincRNAs
  13. Bacterial ncRNAs and riboswitches
  14. CRISPRs


Exercises:

  1. Bioinformatic discovery of miRNA genes
  2. How to Scan and Pick Targets
  3. A case study of miRNA in development
  4. A case study of miRNA function in human disease
  5. Alternative splicing analysis
  6. Computational approaches to characterization of non-coding RNA in bacteria

 

Learning Outcomes

Upon successful completion of this course students should be able to:

  1. Understand the multiple layers of RNA regulation including microRNAs, alternative splicing, riboswitches etc.
  2. Demonstrate proficiency in the recent literature in the filed.
  3. Incorporate RNA studies into his/her own research.

Reading List

N/A

Website

N/A