Course Identification

The theoretical basis of common lab techniques
20183222

Lecturers and Teaching Assistants

Prof. Ari Elson, Dr. Yishai Levin, Dr. Ruti Kapon, Prof. Gideon Schreiber
N/A

Course Schedule and Location

2018
Second Semester
Thursday, 09:15 - 11:00, WSoS, Rm C
15/03/2018

Field of Study, Course Type and Credit Points

Life Sciences: Lecture; Elective; 2.00 points
Life Sciences (Systems Biology Track): 2.00 points
Life Sciences (Molecular and Cellular Neuroscience Track): 2.00 points
Life Sciences (Brain Sciences: Systems, Computational and Cognitive Neuroscience Track): 2.00 points

Comments

N/A

Prerequisites

No

Restrictions

40

Language of Instruction

English

Registration by

16/04/2018

Attendance and participation

Required in at least 80% of the lectures

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

20%
80%

Evaluation Type

Seminar

Scheduled date 1

N/A
N/A
-
N/A

Estimated Weekly Independent Workload (in hours)

2

Syllabus

15.03.18  - Basic design principles for experiments. Biological buffers, solution conditions.  

22.03.18 - Gel electrophoresis, native gels, Western blotting, quantitation of DNA, RNA, protein

29.03.18 - Tissue culture: chemical and biological bases for this method. 

26.4.18 -  Tissue culture: chemical and biological bases for this method. 

10.5.18 -  Basic principles of protein production and purification

17.5.18 -  Methods for analyzing proteins and protein interactions. 

24.5.18 -  Mass spectrometry

31.5.18  -  Mass spectrometry/microscopy

7.6.18  -   Microscopy

14.6.18 -  Students seminar

21.6.18- Students seminar

28.6.18 - Students seminar

5.7.18- Last meeting

 

  • Depending on the number of students registered, we may need to add 1-2 more meetings during the semester. 
  • There will be no exam. However, during the semester two homework exercises will be handed out. Each will count for 10% of the final mark. 
  • In addition, students will also be asked to present (in pairs) seminars about an experimental technique that they choose (following approval by the course instructors). The mark obtained for this presentation will count for 80% of the final grade.

 

Learning Outcomes

Upon successful completion of this course students should be able to:

  1. Demonstrate understanding of the principles in designing an experiment.
  2. Demonstrate knowledge of experimental conditions, including biological buffers, solution conditions and the role of additives.
  3. Recall the correct practices used in tissue culture.
  4. Discuss the basic principles of mass spectrometry, and how to obtain the best results form this method.
  5. Separate and purify biological materials, including proteins.
  6. Demonstrate understanding of the basic principles of microscopy. 

Reading List

N/A

Website

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