Course Identification

Title:

Analysis and sorting by flow and image cytometry

Code:

20183282

Lecturers and Teaching Assistants

Lecturers:

Dr. Ayala Sharp, Mr. Eitan Ariel, Dr. Ziv Porat, Dr. Tomer Meir Salame

TA's:

N/A

Course Schedule and Location

Year:

2018

Semester:

Second Semester

When / Where:

27-31/5/2018 De Picciotto meeting room 25,

First Lecture:

27/05/2018

End date:

31/05/2018

No. of hours (laboratories):

30

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; 0.50 points
Life Sciences (Systems Biology Track): 0.50 points
Life Sciences (Molecular and Cellular Neuroscience Track): 0.50 points

Comments

27-31/05/18
lectures: De Picciotto meeting room 25. Every day from 9-10.30.
Lab: Flow Cytometry lab, Wolfson, room 21.
The lab sessions are: morning-10.45-13.00, afternoon-13.46-16.00.

Prerequisites

No

Restrictions

Participants:

24

Language of Instruction

English

Registration by

Registration By:14/04/2018

Attendance and participation

Obligatory

Grade Type

Pass / Fail

Grade Breakdown (in %)

Attendance

100%

Evaluation Type

Laboratory

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

N/A

Syllabus

Principles of operation of Flow and Image Cytometers: the flow systems, sample excitation and illumination, sample detectors.
Applications of Flow and Image Cytometry: measurement of multiple color staining, DNA measurements, Nuclear - cytoplasm translocations.
Cell Sorting and analysis of yield and purity.
Preparation of samples.
Data analysis.

Learning Outcomes

Upon successful completion of this course students should be able to:

Discuss the principles and some applications of Flow and Image Cytometry.
Run samples on Flow and Image Cytometers.
Analyze samples with multi-color staining.
Sort multi-color cell populations to test tubes.
Perform cell cycle analysis.

Reading List

Shapiro H. M - Practical Flow Cytometry.
Givan A. L - Flow Cytometry first principles.
Ormerod M. G. - Flow Cytometry

Website

N/A