Course Identification

Title:

Protein purification, crystallisation and structural analysis

Code:

20183402

Lecturers and Teaching Assistants

Lecturers:

Dr. Shira Albeck, Dr. Orly Dym, Mr. Jossef Jacobovitch, Ms. Shelly Hamer-Rogotner, Maya Kashani

TA's:

N/A

Course Schedule and Location

Year:

2018

Semester:

Second Semester

When / Where:

3-7/6, FGS, Lab

First Lecture:

03/06/2018

End date:

07/06/2017

No. of hours (laboratories):

25

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; 0.50 points
Chemical Sciences: 0.50 points
Life Sciences (Systems Biology Track): 0.50 points
Life Sciences (Molecular and Cellular Neuroscience Track): 0.50 points

Comments

N/A

Prerequisites

No

Restrictions

Participants:

20

Language of Instruction

English

Attendance and participation

Obligatory

Grade Type

Pass / Fail

Grade Breakdown (in %)

Attendance

100%

Evaluation Type

Laboratory

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

3

Syllabus

Bioinformatic analysis of proteins.
Protein purification using: affinity, ion exchange, size exclusion.
Protein analysis: removal of tags, SDS PAGE, dialysis, concentration of proteins and methods to determine protein concentration.
Protein size and protein-protein interactions: size exclusion and native gels.
Crystallization of proteins
3D structure determination and preliminary structure analysis.

Learning Outcomes

Upon successful completion of this course students should be able to:

Demonstrate basic knowledge and experience in protein bioinformatics, purification, analysis, crystallization, and 3D structure determination.

Reading List

N/A

Website

N/A