Course Identification

Title:

Protein purification, crystallisation and structural analysis

Code:

20193352

Lecturers and Teaching Assistants

Lecturers:

Dr. Shira Albeck, Dr. Orly Dym, Maya Kashani

TA's:

N/A

Course Schedule and Location

Year:

2019

Semester:

Second Semester

When / Where:

March 24-28, 2019; 10:00-16:00 , FGS, Lab

First Lecture:

24/03/2019

End date:

28/03/2019

No. of hours (laboratories):

20

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; 0.50 points
Life Sciences (Molecular and Cellular Neuroscience Track): Laboratory; Elective; 0.50 points
Life Sciences (Computational and Systems Biology Track): Laboratory; Elective; 0.50 points

Comments

N/A

Prerequisites

No

Restrictions

Participants:

20

Language of Instruction

English

Registration by

Registration By:15/02/2019

Attendance and participation

Obligatory

Grade Type

Pass / Fail

Grade Breakdown (in %)

Attendance

100%

Evaluation Type

Laboratory

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

3

Syllabus

Bioinformatic analysis of proteins.
Protein purification using: affinity, ion exchange, size exclusion, hydrophobic interaction, AS precipitation, refolding IBs.
Protein analysis: removal of tags, SDS PAGE, dialysis, concentration of proteins and methods to determine protein concentration.
Protein size and protein-protein interactions: size exclusion and native gels.
Crystallization of proteins
3D structure determination and preliminary structure analysis.

Learning Outcomes

Upon successful completion of this course students should be able to:

Demonstrate basic knowledge and experience in protein bioinformatics, purification, analysis, crystallization, and 3D structure determination.

Reading List

N/A

Website

N/A