Course Identification

Analysis and sorting by flow andimage cytometry
20193372

Lecturers and Teaching Assistants

Dr. Ziv Porat, Dr. Tomer Meir Salame, Dr. Ekaterina Kopitman, Dr. Efrat Hagai
N/A

Course Schedule and Location

2019
Second Semester
2/6-6/6 2019; 9:00-16:00; Venue- see comments,
02/06/2019
30

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; 0.50 points
Life Sciences (Molecular and Cellular Neuroscience Track): Laboratory; Elective; 0.50 points
Life Sciences (Computational and Systems Biology Track): Laboratory; Elective; 0.50 points

Comments

Lecture 9:00-10:30 at
Candiotty seminar room, lab at Wolfson room 21, 10:45-13:00 (morning group) or 13:45-16:00 (afternoon group)

Prerequisites

No

Restrictions

24

Language of Instruction

English

Registration by

23/05/2019

Attendance and participation

Obligatory

Grade Type

Pass / Fail

Grade Breakdown (in %)

100%

Evaluation Type

Laboratory

Scheduled date 1

N/A
N/A
-
N/A

Estimated Weekly Independent Workload (in hours)

N/A

Syllabus

  1. Principles of operation of Flow and Image Cytometers: the flow systems, sample excitation and illumination, sample detectors.
  2. Applications of Flow and Image Cytometry: measurement of multiple color staining, DNA measurements, Nuclear - cytoplasm translocations.
  3. Cell Sorting and analysis of yield and purity.
  4. Preparation of samples.
  5. Data analysis.

Learning Outcomes

Upon successful completion of this course students should be able to:

  1. Discuss the principles and some applications of Flow and Image Cytometry.
  2. Run samples on Flow and Image Cytometers.
  3. Analyze samples with multi-color staining.
  4. Sort multi-color cell populations to test tubes.
  5. Perform cell cycle analysis.

Reading List

  1. Shapiro H. M - Practical Flow Cytometry.
  2. Givan A. L - Flow Cytometry first principles.
  3. Ormerod M. G. - Flow Cytometry

Website

N/A