Course Identification

Low molecular weight biological molecules or so called ‘metabolites’ are vital for viability and fitness of all living creatures. Detection, identification and quantification of hundreds of thousands of metabolites possessing endless structures is a great challenge, requiring ample expertise in analytical chemistry. Yet, proficiency in such methodologies is critically needed in many labs, both biology and chemistry oriented ones. This course will offer a unique opportunity for scientists to gain fundamental knowledge in using analytical chemistry for the interrogation of metabolites in any given organism.

Chromatography and mass spectrometry are the core technologies that provide the capability to measure metabolites. The course will provide both theoretical background as well as practical experience with respect to analyzing small molecules in complex extracts. Course attendees will get acquainted with cutting edge liquid and gas chromatography coupled to mass spectrometry technologies (i.e. LC-MS & and GC-MS). The practical part will include hands-on activity (working in small groups) that will cover sample preparation, analytical runs on advanced LC-MS and GC-MS systems and raw data analysis as well as downstream data processing.

Upon successful completion of this course students should be able to:

1. Demonstrate understanding of the basics of liquid and gas chromatography.
2. Demonstrate understanding of the basics of mass spectrometry.
3. Prepare samples for the different methods of analysis, appreciate data collection and analysis.
4. Discuss the various applications, limitations and restrictions of the various methods.