The course will expose students to advanced techniques in immunology and their application in specific experimental systems investigated in our department. It will cover both background and historical aspects of each technique, include specific examples from the literature and will also present innovative implementations of these methods by specific setups currently in use by individual labs in the department.
1
course overview+Brief and advanced introduction to the immune system
2
Microfluidics
3
Novel FACS applications for ex vivo analysis
4
Image stream
5
High throughput sequencing and analysis
6
Advanced in vivo methods
7
Two photon microscopy
8
Novel Cytoff applications for ex vivo analysis
9
10
Two photon and light sheet microscopy
11
Transgenic, reporter and knockout mice
12
CRISPR
13
Antibody production and application
14
High-throughput analysis of TCR and BCR repertoires
15
Proteomics in immunology
Upon successful completion of this course students should be able to: