Course Identification

The theoretical basis of common lab techniques
20203412

Lecturers and Teaching Assistants

Prof. Ari Elson, Prof. Gideon Schreiber, Dr. Tali Dadosh, Dr. Dan Michael
Dr. Maya Shemesh

Course Schedule and Location

2020
Second Semester
Tuesday, 11:15 - 13:00, WSoS, Rm C
21/04/2020

Field of Study, Course Type and Credit Points

Life Sciences: Lecture; Elective; Regular; 2.00 points
Chemical Sciences: Lecture; Elective; Regular; 2.00 points
Life Sciences (Molecular and Cellular Neuroscience Track): Lecture; Elective; Regular; 2.00 points
Life Sciences (Brain Sciences: Systems, Computational and Cognitive Neuroscience Track): Lecture; Elective; Regular; 2.00 points
Life Sciences (Computational and Systems Biology Track): Lecture; Elective; Regular; 2.00 points

Comments

Will be taught via Zoom starting April 19th.

Prerequisites

No

Restrictions

40

Language of Instruction

English

Attendance and participation

Required in at least 80% of the lectures

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

20%
80%

Evaluation Type

Seminar

Scheduled date 1

N/A
N/A
-
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Estimated Weekly Independent Workload (in hours)

2

Syllabus

21.04.20 - Basic design principles for experiments. Biological buffers, solution conditions.  (Gideon Schreiber)

28.04.20 - No classes: Yom Hazikaron

05.05.20 - Basic principles of protein production and purification (Gideon Schreiber)

12.05.20 - No classes: Lag Baomer

19.05.20 - Gel electrophoresis, native gels, Western blotting, quantitation of DNA, RNA, protein (Ari Elson)

26.05.20 - Methods for analyzing proteins and protein interactions. (Gideon Schreiber)

02.06.20 - Tissue culture: chemical and biological bases for this method. (Ari Elson)

09.06.20 - PCR (Dan Michael)

16.06.20 - Microscopy (Tali Dadosh)

23.06.20 - Microscopy (Tali Dadosh)

30.06.20 - Students seminar (Gideon Schreiber & Ari Elson)

07.07.20 - Students seminar (Gideon Schreiber & Ari Elson)

14.07.20 - Students seminar (Gideon Schreiber & Ari Elson)

21.07.20 - Students seminar (Gideon Schreiber & Ari Elson)

Learning Outcomes

Upon successful completion of this course students should be able to:

  1. Demonstrate understanding of the principles in designing an experiment.
  2. Demonstrate knowledge of experimental conditions, including biological buffers, solution conditions and the role of additives.
  3. Recall the correct practices used in tissue culture.
  4. Discuss the basic principles of mass spectrometry, and how to obtain the best results form this method.
  5. Separate and purify biological materials, including proteins.
  6. Demonstrate understanding of the basic principles of microscopy. 

Reading List

N/A

Website

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