Course Identification

Analysis and sorting by flow and image cytometry- Theory
20213141

Lecturers and Teaching Assistants

Dr. Ziv Porat, Dr. Tomer Meir Salame, Dr. Efrat Hagai, Dr. Ekaterina Kopitman
N/A

Course Schedule and Location

2021
First Semester
There will be daily lectures - 9:00-10:30. The lab will be held for 5 days in two groups - 11:00-12:30 and 13:30-15:00,
14/01/2021
24/01/2021
30

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; Regular; 0.50 points
Life Sciences (Molecular and Cellular Neuroscience Track): Laboratory; Elective; Regular; 0.50 points
Life Sciences (Computational and Systems Biology Track): Laboratory; Elective; Regular; 0.50 points

Comments

The ZOOM lectures are open to all students, but participants in the wet lab will be chosen according to their experience and need for utilizing Flow Cytometry for their research.
14/1, 17-21/1? 9:00-10:30 ? lectures
17-21/1/21 ? Wet lab sessions in two groups, 6 students/session: 11:00-12:30, 13:30-15:00 (2 instructors, total of 30 teaching hours)
24/1/21 ? Online demonstration of Imaging flow Cytometry analysis ? 9:00-11:00.

Prerequisites

No

Restrictions

12

Language of Instruction

English

Attendance and participation

Obligatory

Grade Type

Pass / Fail

Grade Breakdown (in %)

50%
30%
20%

Evaluation Type

Laboratory

Scheduled date 1

N/A
N/A
-
N/A

Estimated Weekly Independent Workload (in hours)

2

Syllabus

  1. Principles of operation of Flow and Image Cytometers: the flow systems, sample excitation and illumination, sample detectors.
  2. Applications of Flow and Image Cytometry: measurement of multiple color staining, DNA measurements, Nuclear - cytoplasm translocations.
  3. Cell Sorting and analysis of yield and purity.
  4. Preparation of samples.
  5. Data analysis.

Learning Outcomes

Upon successful completion of this course students should be able to:

  1. Discuss the principles and some applications of Flow and Image Cytometry.
  2. Run samples on Flow and Image Cytometers.
  3. Analyze samples with multi-color staining.
  4. Sort multi-color cell populations to test tubes.
  5. Perform cell cycle analysis.

Reading List

  1. Shapiro H. M - Practical Flow Cytometry.
  2. Givan A. L - Flow Cytometry first principles.
  3. Ormerod M. G. - Flow Cytometry

Website

N/A