Course Identification

Title:

Protein purification, crystallization and structural analysis

Code:

20223032

Lecturers and Teaching Assistants

Lecturers:

Dr. Shira Albeck, Dr. Orly Dym, Dr. Yael Fridmann-Sirkis, Dr. Moshe Goldsmith, Ms. Shelly Hamer-Rogotner, Dr. Yael Galon Wolfenson

TA's:

ג'ושוע בירבאום, Jossef Eyal Jacobovitch

Course Schedule and Location

Year:

2022

Semester:

Second Semester

When / Where:

9:00-16:00, FGS lab, FGS, Lab

First Lecture:

03/07/2022

End date:

13/07/2022

No. of hours (laboratories):

20

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; Regular; 1.00 points
Chemical Sciences: Laboratory; 1.00 points
Life Sciences (Molecular and Cellular Neuroscience Track): Laboratory; 1.00 points
Life Sciences (Computational and Systems Biology Track): Laboratory; 1.00 points

Comments

Second session: 3-7.7.2022 - Shira Albeck & Yael Fridmann Sirkis
3.7-7.7 & 11.7-13.7

Prerequisites

No

Restrictions

Participants:

30

Language of Instruction

English

Registration by

Registration By:27/04/2022

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

Attendance

100%

Evaluation Type

Final assignment

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

3

Syllabus

First session: 15 - 17.5.2022 - (9:00-16:00) - Orly Dym

”Protein crystallization, structure analysis, modeling and visualization by PyMOL”

· Bioinformatic tools for protein analysis.

· Protein crystallization – techniques, strategies and tips.

· Correlation between proteins sequence-function-structure.

· Protein structure prediction.

· Protein visualization – PyMol

Second session: 3 - 7.7.2022 - (9:00-16:00) - Shira Albeck & Yael Fridmann Sirkis

“Protein purification, characterization & protein-protein interaction analysis”

Protein purification using a variety of tags & biophysical properties
Handling purified proteins:

Concentrating proteins

Buffer exchange

Labeling

Determining protein concentration

Protein stabilization

Analysis of protein:

Oligomeric state by SEC

Stability by DSF

Analysis of protein-protein interaction: SEC, ICP, MST & Biacore

Learning Outcomes

Upon successful completion of this course students should be able to:

Demonstrate basic knowledge and experience in protein bioinformatics, purification, analysis, crystallization, and 3D structure determination.

Reading List

N/A

Website

N/A