Course Identification

Application of live cell microscopy methods to study intracellular trafficking and organelle dynamics.
20223301

Lecturers and Teaching Assistants

N/A

Course Schedule and Location

2022
First Semester
Sunday, 11:15 - 13:00, WSoS, Rm A
24/10/2021
18/03/2022

Field of Study, Course Type and Credit Points

Life Sciences: Lecture; Elective; Regular; 2.00 points
Life Sciences (Molecular and Cellular Neuroscience Track): Lecture; Elective; Regular; 2.00 points

Comments

For each session participants are required to read the manuscript that will be discussed.

Prerequisites

A basic course in general cell biology

Restrictions

40

Language of Instruction

English

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

100%

Evaluation Type

Examination

Scheduled date 1

02/03/2022
WSoS, Rm A
1000-1200
N/A

Scheduled date 2

15/03/2022
WSoS, Rm A
1000-1200
N/A

Estimated Weekly Independent Workload (in hours)

N/A

Syllabus

The course will begin with 2 introductory lectures. From then on, it will be based on selected papers that cover various aspects of Intracellular trafficking. The course focuses on two topics: 

1. Scientific: Major advances in the field of Intracellular trafficking. 

2. Technical: The use of microscopy methods such as Fluorescence Recovery After Photobleaching. FRET. Combining live cell analysis with advanced quantitative approaches (Kinetic modeling and simulation) Super-resolution microscopy, etc.

Participants are required to read the papers in advance to promote interactive high-level discussions during the lectures. 

A partial list of titles covered:   

Quantitative analysis of Secretory transport

The whereabouts of the Golgi apparatus during mitosis

Unboxing the Golgi using microscopy-based kinetic analysis tools

Mechanisms of cargo protein selection, concentration, and export from the ER

From photoactivated fluorescent protein to super-resolution microscopy (A zoom recording of Dr. George H. Patterson)

Unboxing the Golgi

Viral cell biology: HCV and lipid droplets

 

Learning Outcomes

Gain some updates on the Intracellular trafficking field. Learn about the strengths of various microscopy approaches especially live-cell microscopy. Learn about controversies in science. Enjoy some nice images and movies of living cells

Reading List

will be updated during the course

Website

N/A