Course Identification

Title:

Seminar in mRNA trafficking and localization in development & disease

Code:

20223222

Lecturers and Teaching Assistants

Lecturers:

Dr. Gal Haimovich, Prof. Jeffrey Gerst

TA's:

N/A

Course Schedule and Location

Year:

2022

Semester:

Second Semester

When / Where:

Wednesday, 14:15 - 16:00, Belfer, Koshland Rm

First Lecture:

30/03/2022

End date:

19/08/2022

Field of Study, Course Type and Credit Points

Life Sciences: Seminar; Elective; Regular; 2.00 points
Life Sciences (Molecular and Cellular Neuroscience Track): Seminar; 2.00 points
Life Sciences (Computational and Systems Biology Track): Seminar; 2.00 points

Comments

Location: Koshland auditorium (Aquarium) in Belfer.

Prerequisites

Knowledge in molecular biology.

Restrictions

Participants:

13

Language of Instruction

English

Registration by

Registration By:27/03/2022

Attendance and participation

Required in at least 80% of the lectures

Grade Type

Pass / Fail

Grade Breakdown (in %)

Seminar:

100%

presenting a paper during the semester, and attending at least 80% of the meetings.

Evaluation Type

Seminar

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

2

Syllabus

The 1st session will be dedicated to an introduction and an overview of the topic:

Why localize RNA?

Methods – biochemical & imaging (FISH, MS2-like, GFP-mimic RNA aptamers, imaging local translation); large-scale analysis

mRNA localization in yeast & mammals as a platform to study the concepts of mRNA localization & local translation regulation.

mRNA trafficking in model organisms and consequences to physiology

mRNA localization to organelles

Extracellular RNA and implications to cancer research

A list of papers will be distributed on the first lecture. Each student will present one paper from the list. Students are expected to read the papers and participate in the discussion.

Learning Outcomes

Upon successful completion of the course, the student will be able to:

Read critically and discuss the current literature related to mRNA trafficking and localization in development & disease.
Demonstrate knowledge of cutting-edge topics in the field.
Manifest familiarity with state-of-the-art methods in the field.

Reading List

Introduction & overview:

Buxbaum AR, Haimovich G. & Singer RH (2015) In the right place at the right time: visualizing and understanding mRNA localization. Nature Reviews Molecular Cell Biology 16, 95–109
Jansen RP, Niessing D, Baumann S, and Feldbrugge M (2014) mRNA transport meets membrane traffic. Trends Genet 30, 408-417

A list of papers to present will be distributed on the first lecture.

Website

N/A