Course Identification

Title:

Protein purification, protein-protein interactions and crystallization & structural analysis

Code:

20243042

Lecturers and Teaching Assistants

Lecturers:

Dr. Shira Albeck, Dr. Orly Dym, Dr. Yael Fridmann-Sirkis, Ms. Shelly Hamer-Rogotner, Mr. Jossef Jacobovitch, Ms. Hedva Hamawi, Dr. Irina Shin, Dr. Moshe Goldsmith, Dr. Eliane Hadas Yardeni

TA's:

N/A

Course Schedule and Location

Year:

2024

Semester:

Second Semester

When / Where:

09:00 - 16:00, WSoS, Lab

First Lecture:

30/06/2024

End date:

09/07/2024

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; 1.00 points
Chemical Sciences: Laboratory; Elective; Regular; 1.00 points
Life Sciences (ExCLS Track): Elective; 1.00 points

Comments

June 30-July 4th: Protein purification and characterisation (Shira Albeck & Yael Fridmann Sirkis)
July7-July 9th: Protein crystallization and structure analysis (Orly Dym)

Hours (daily): 9:00-16:00
Location: FGS lab (ground floor) + adjacent lecture room
In-person course

Prerequisites

N/A

Restrictions

Participants:

No

Language of Instruction

English

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

Final:

100%

Evaluation Type

Laboratory

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

3

Syllabus

”Protein crystallization, structure analysis, modeling and visualization by PyMOL”

· Bioinformatic tools for protein analysis.

· Protein crystallization – techniques, strategies and tips.

· Correlation between proteins sequence-function-structure.

· Protein structure prediction.

· Protein visualization – PyMol

“Protein purification, characterization & protein-protein interaction analysis”

Protein purification using a variety of tags & biophysical properties
Handling purified proteins:

Concentrating proteins

Buffer exchange

Labeling

Determining protein concentration

Protein stabilization

Analysis of protein:

Oligomeric state by SEC

Stability by DSF & DLS

Analysis of protein-protein interaction: ITC, MST & Biacore

Lab course schedule: Protein purification & Analysis

Sunday

9:00 – 16:00 Protein purification lectures + experiments

Monday

9:00 – 16:00 Protein purification lectures + experiments

Tuesday

9:00 - 14:00 Protein analysis

14:00 - 16:00 Protein purification lecture + experiment

Wednesday

9:00 - 10:30 Protein purification lab

10:30-13:00 Protein purification exersize

13:00 - 16:00 Protein analysis lecture + experiment

Thursday

9:00 - 13:30 Protein purification lecture + experiment

13:30 - 16:00 Protein analysis experiment

Sunday

9:00 – 16:00 Protein crystallization lectures & experiment

Monday

9:00 – 16:00 Protein crystallization lectures & experiment

Tuesday

9:00 – 13:00 Protein fold anaylsis Pymol

13:00 – 16:00 Exam

Learning Outcomes

Upon successful completion of this course students should be able to:

Demonstrate basic knowledge and experience in protein bioinformatics, protein purification, analysis of protein-protein interactions, crystallization of proteins and 3D structure analysis.

Reading List

N/A

Website

N/A