This course is designed to provide students with a comprehensive understanding of advanced light microscopy techniques and their practical applications in various scientific fields. The course includes both theoretical lectures and hands-on experience in Weizmann microscopy cores/ departmental cores.
Part 1: Lectures (11 weeks, 2h lecture)
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Date (Tuesday, 11:00 - 13:00)
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Lecture name
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Given by
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1
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09.04.2024
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Microscopy basics
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Sefi Addadi
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2
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16.04.2024
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Fluorescence Microscopy
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Tali Dadosh
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3
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30.04.2024
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Fluorophores and Fluorescent Proteins
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Inna Goliand
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4
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07.05.2024
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Camera and digital image
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Tatiana Smirnova
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5
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21.05.2024
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Optical Sectioning and Confocal Microscopy
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Reinat Nevo
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6
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28.05.2024
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Specimen Preparation
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Inna Goliand
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30.05.2024
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7
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04.06.2024
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Image analysis considerations for imaging
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Ofra Golani
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8
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18.06.2024
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Advanced imaging methods 1
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Reinat Nevo
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9
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25.06.2024
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Advanced imaging methods 2
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Sefi Addadi
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10
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02.07.2024
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Advanced imaging methods 3
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Tatiana Smirnova + Tali Dadosh
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11
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09.07.2024
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Choosing a Microscope Platform and Core Imaging Facilities
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Sefi Addadi
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Part 2: Hands-On Training (4 partial days)
Hands-on dates will be coordinated during the course,
Day 1 will be carried out at the mid of the course.
Day 2 and 3 will be sometime in June/July dates will be determined by pre-registration
Day 1 (4H): Hands-on sessions in the microscopy core facilities
- Demo of basic microscopy concept
- Image acquisition and optimization
- Image visualization
Day 2 (5H): Hands –on short project on a specific microscope (Bring your samples!)
Day 3 (4H): Advanced methods demo
- Light sheet microscopy
- Two-photon microscopy
- STED
- STORM
- FRAP-FRET
- FLIM
- TIRF
- Multiplexing
Day 4 (3H): Discussion and home assignment presentation.