Course Identification

The 1^st session will be dedicated to an introduction and an overview of the topic: 

• Why localize RNA?
• Methods – biochemical vs imaging (single molecule FISH, multiplex FISH and sequencing FISH,  MS2-like systems, GFP-mimic RNA aptamers, single molecule translation imaging)
• mRNA trafficking in model organisms
• mRNA localization to organelles and RNA granules
• Novel concepts of localized translation
• Extracellular and transferred RNA 

A list of recent research papers will be distributed on the first lecture.

Students are expected to read all the papers and participate in the discussion.

Each student will present one paper from the list.

Upon successful completion of the course, the student will be able to:
1. Read critically and discuss the current literature related to RNA localization and local translation.
2. Demonstrate knowledge of cutting-edge topics in the field.

Introduction & overview:

Das S, Vera M, Gandin V, Singer RH, Tutucci E. Intracellular mRNA transport and localized translation. Nat Rev Mol Cell Biol. 2021 Jul;22(7):483-504

Buxbaum AR, Haimovich G. & Singer RH (2015) In the right place at the right time: visualizing and understanding mRNA localization. Nature Reviews Molecular Cell Biology Volume:16, Pages:95–109 

A list of papers to present will be distributed on the first lecture.