Course Identification

Title:

Advanced light microscopy

Code:

20253052

Lecturers and Teaching Assistants

Lecturers:

Dr. Inna Goliand, Dr. Yoseph Addadi, Dr. Tali Dadosh, Dr. Reinat Nevo, Ms. Ofra Golani, Dr. Eyal Schejter, Dr. Melanie Horev, Ms. Tatiana Smirnova

TA's:

N/A

Course Schedule and Location

Year:

2025

Semester:

Second Semester

When / Where:

Tuesday, 11:15 - 13:00, Ulman, 101

First Lecture:

25/03/2025

End date:

01/07/2025

No. of hours (laboratories):

18

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; Regular; 2.00 points
Life Sciences (Brain Sciences: Systems, Computational and Cognitive Neuroscience Track): Laboratory; 2.00 points

Comments

N/A

Prerequisites

No

Restrictions

Participants:

28

Language of Instruction

English

Registration by

Registration By:24/11/2024

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

Attendance

35%

Assignments:

15%

Seminar:

50%

Evaluation Type

Seminar

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

2

Syllabus

This course is designed to provide students with a comprehensive understanding of advanced light microscopy techniques and their practical applications in various scientific fields. The course includes both theoretical lectures and hands-on experience in Weizmann microscopy cores/ departmental cores.

Lectures

Date (Tuesday, 11:15-13:00)	Lecture Name	Given By
25.3.25	Microscopy Basics	Sefi Addadi
1.4.25	Fluorescence Microscopy + Camera and Digital Image + Phase Contrast and Bright Field Microscopy	Tatiana Smirnova + Tali Dadosh
8.4.25	Fluorophores and Fluorescent Proteins	Inna Goliand
22.4.25	Short Intro + First Hands-on Phase Contrast and Bright Field Microscopy	All Instructors
29.4.25	Optical Sectioning and Confocal Microscopy	Reinat Nevo
13.5.25	Specimen Preparation	Inna Goliand
20.5.25	Image Analysis Considerations for Imaging	Ofra Golani
3.6.25	Advanced Imaging Methods 1	Reinat Nevo
10.6.25	Advanced Imaging Methods 2	Sefi Addadi
17.6.25	Advanced Imaging Methods 3	Tatiana Smirnova + Tali Dadosh
24.6.25	Choosing a Microscope Platform and Core Imaging Facilities	Sefi Addadi

Hands-On

Date	Topic	Given By
Week of 4.5.25	WF vs. Confocal - General Concepts	All Instructors
Week of 25.5.25	Student Samples	All Instructors
1.7.25	Advanced Methods	All Instructors
TBD	Final Assignment Presentation	All Instructors

Learning Outcomes

Upon successful completion of the course, students will be able to:

Understand the principles and operation of advanced light microscopes.
Select appropriate sample preparation techniques based on the specific research or imaging goals.
Understand the advantages and limitations of various imaging modes.
Plan and design experiments that incorporate advanced light microscopy as a central technique.
Understand the importance of control experiments and appropriate controls in microscopy research.
Understanding basic concepts of quantification of images.
Apply critical thinking and problem-solving skills to address challenges in microscopy and related research projects.

Reading List

iBiology Microscopy Short Course

https://www.youtube.com/watch?v=4c5ILWQmqRY&list=PLQFc-Dxlf4pSRaEk8Xi9BzS0r8-LYmwRQ&ab_channel=iBiologyTechniques

A beginner’s guide to rigor and reproducibility in fluorescence imaging experiments

https://www.molbiolcell.org/doi/full/10.1091/mbc.E17-05-0276

Community-developed checklists for publishing images and image analyses

https://pubmed.ncbi.nlm.nih.gov/37710020/

Microcourses – Jennifer Waters

https://www.youtube.com/microcourses

Website

N/A