Course Identification

Title:

Protein purification, protein-protein interactions and protein crystallization & structural analysis

Code:

20253202

Lecturers and Teaching Assistants

Lecturers:

Dr. Shira Albeck, Dr. Orly Dym, Dr. Yael Fridmann-Sirkis, Ms. Shelly Hamer-Rogotner, Mr. Jossef Jacobovitch, Ms. Hedva Hamawi, Dr. Irina Shin, Dr. Moshe Goldsmith, Dr. Eliane Hadas Yardeni

TA's:

N/A

Course Schedule and Location

Year:

2025

Semester:

Second Semester

When / Where:

09:00 - 16:00, WSoS, Lab

First Lecture:

11/05/2025

End date:

20/05/2025

No. of hours (laboratories):

56

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; 1.00 points
Chemical Sciences: Laboratory; 1.00 points

Comments

In-person lab course

The course will be given between 11-20 of May 2025 daily between 9-16:00.
Between 11-15th of May 2025, the course will be given daily at the FGS lab and adjacent classroom (coordinated with Danny).
On Wednesday (14.5) the activity will take place in the labs of the LSCF
18/5/2025 from 9:00-10:00 and from 13:00-14:00- WSoS ROOM A
19/5/2025 from 9:00-10:00 and from 12:00-13:00- WSoS ROOM B

Prerequisites

No

Restrictions

Participants:

25

Language of Instruction

English

Registration by

Registration By:24/02/2025

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

Final:

100%

complete attendance required

Evaluation Type

Examination

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Scheduled date 2

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

4

Syllabus

”Protein crystallization, structure analysis, modeling and visualization by PyMOL”

Bioinformatic tools for protein analysis.

Protein crystallization – techniques, strategies and tips.

Correlation between proteins sequence-function-structure.

Protein structure prediction.

Protein visualization – PyMol

“Protein purification, characterization & protein-protein interaction analysis”

Protein purification using a variety of tags & biophysical properties

Columns & FPLC

Handling purified proteins:

Concentrating proteins

Buffer exchange

Labeling

Determining protein concentration

Protein stabilization

Analysis of protein oligomeric state by SEC

Analysis of protein-protein interaction: ITC, MST & Biacore

Analysis of protein stability by DSF & DLS

Learning Outcomes

Upon completion of this course students should be able to demonstrate basic knowledge and experience in protein bioinformatics, protein purification, analysis of protein-protein interactions, crystallization of proteins and 3D structure analysis.

Reading List

N/A

Website

N/A