Course Identification

Analysis and sorting by flow, image and mass cytometry
20253021

Lecturers and Teaching Assistants

Dr. Ziv Porat, Dr. Tomer Meir Salame, Dr. Ekaterina Kopitman
N/A

Course Schedule and Location

2025
First Semester
An introductory lecture on the 9/1/25, and the lab course on 12-16/1/2025.,
09/01/2025
16/01/2025

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; 1.00 points

Comments

9/1/2025: 9:00-10:30 Introductory lecture Ullman
12-14/1/2025 & 16/1/2025: 9:00-10:30 lecture Ullman
10:45-13:00 lab session (morning group) Ullman
13:45-16:00 lab session (Afternoon group) Ullman
15/1/25: 09:00-10:30 lecture FGS Room C
10:45-13:00 lab session (morning group) Ullman
13:45-16:00 lab session (Afternoon group) Ullman
19/1/2025: 9:00-16:00 CyTOF lab demonstration Ullman

Prerequisites

No

Restrictions

30

Language of Instruction

English

Registration by

06/11/2024

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

60%
40%

Evaluation Type

Examination

Scheduled date 1

09/02/2025
WSoS, Rm C
0900-1200
N/A

Scheduled date 2

02/03/2025
Ulman, 101
0900-1200
N/A

Estimated Weekly Independent Workload (in hours)

3

Syllabus

  1. Principles of operation of Flow, Image and Mass Cytometers: the flow systems, sample excitation and emission, sample detectors.
  2. Applications of Flow, Image and Mass Cytometry: measurement of multiple markers staining, DNA measurements.
  3. Cell Sorting and analysis of yield and purity.
  4. Preparation of samples.
  5. Data analysis.

Learning Outcomes

Upon successful completion of this course students should be able to:

  1. Discuss the principles applications of Flow, Image and Mass Cytometry.
  2. Run samples on Flow and Image Cytometers.
  3. Analyze samples with multi-marker staining.
  4. Sort multi-marker cell populations to test tubes.
  5. Perform cell cycle analysis.

Reading List

  1. Shapiro H. M - Practical Flow Cytometry.
  2. Givan A. L - Flow Cytometry first principles.
  3. Ormerod M. G. - Flow Cytometry.
  4.  CyTOF for the Masses (2022) – Lyer A. et al. Frontiers in Immunology.
  5. BD FACSAria III User's Guide (https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/BD_FACSAria_III_User_Guide.pdf)

Website

N/A