Course Identification

Analysis and sorting by flow, image and mass cytometry
20263462

Lecturers and Teaching Assistants

Dr. Ziv Porat, Dr. Tomer Meir Salame, Dr. Ekaterina Kopitman, Ms. Yulia Frug
N/A

Course Schedule and Location

2026
Second Semester
N/A
28/05/2026
04/06/2026

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; 1.00 points

Comments

In-Person lab will be held between 28/5-4/6/26 following the schedule below:
28/5/26:
9:00-10:30 morning lecture at 191 Benoziyo Biochemistry auditorium
The rest of the day will be held at DWEK building, Floor 1 as follows:
10:45-13:00 lab session (morning group)
13:45-16:00 lab session (Afternoon group)
09:00-10:30 lecture
10:45-13:00 lab session (morning group)
13:45-16:00 lab session (Afternoon group)

31/5/26:
9:00-10:30 morning lecture at WSoS, Rm B
The rest of the day will be held at DWEK building, Floor 1 as follows:
10:45-13:00 lab session (morning group)
13:45-16:00 lab session (Afternoon group)
09:00-10:30 lecture
10:45-13:00 lab session (morning group)
13:45-16:00 lab session (Afternoon group)

1/6/26:
9:00-10:30 morning lecture at Drory auditorium, Weissman building
The rest of the day will be held at DWEK building, Floor 1 as follows:
10:45-13:00 lab session (morning group)
13:45-16:00 lab session (Afternoon group)
09:00-10:30 lecture
10:45-13:00 lab session (morning group)
13:45-16:00 lab session (Afternoon group)

2/6/26:
9:00-10:30 morning lecture at WSoS Room C
The rest of the day will be held at DWEK building, Floor 1 as follows:
10:45-13:00 lab session (morning group)
13:45-16:00 lab session (Afternoon group)
09:00-10:30 lecture
10:45-13:00 lab session (morning group)
13:45-16:00 lab session (Afternoon group)

3/6/26
9:00-10:30 morning lecture at 191 Benoziyo Biochemistry auditorium
The rest of the day will be held at DWEK building, Floor 1 as follows:
10:45-13:00 lab session (morning group)
13:45-16:00 lab session (Afternoon group)
09:00-10:30 lecture
10:45-13:00 lab session (morning group)
13:45-16:00 lab session (Afternoon group)

4/6/26
9:00-10:30 morning lecture at 191 Benoziyo Biochemistry auditorium
The rest of the day will be held at DWEK building, Floor 1 as follows:
10:45-13:00 lab session (morning group)
13:45-16:00 lab session (Afternoon group)
09:00-10:30 lecture
10:45-13:00 lab session (morning group)
13:45-16:00 lab session (Afternoon group)


Prerequisites

No

Restrictions

30

Language of Instruction

English

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

60%
40%

Evaluation Type

Examination

Scheduled date 1

N/A
N/A
-
N/A

Scheduled date 2

N/A
N/A
-
N/A

Estimated Weekly Independent Workload (in hours)

3

Syllabus

  1. Principles of operation of Flow, Image and Mass Cytometers: the flow systems, sample excitation and emission, sample detectors.
  2. Applications of Flow, Image and Mass Cytometry: measurement of multiple markers staining, DNA measurements.
  3. Cell Sorting and analysis of yield and purity.
  4. Preparation of samples.
  5. Data analysis.

Learning Outcomes

Upon successful completion of this course students should be able to:

  1. Discuss the principles applications of Flow, Image and Mass Cytometry.
  2. Run samples on Flow and Image Cytometers.
  3. Analyze samples with multi-marker staining.
  4. Sort multi-marker cell populations to test tubes.
  5. Perform cell cycle analysis.

Reading List

  1. Shapiro H. M - Practical Flow Cytometry.
  2. Givan A. L - Flow Cytometry first principles.
  3. Ormerod M. G. - Flow Cytometry.
  4.  CyTOF for the Masses (2022) – Lyer A. et al. Frontiers in Immunology.
  5. BD FACSAria III User's Guide (https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/BD_FACSAria_III_User_Guide.pdf)

Website

N/A