Course Identification

Protein purification, protein-protein interactions and protein crystallization & structural analysis
20263272

Lecturers and Teaching Assistants

Dr. Shira Albeck, Dr. Orly Dym, Dr. Yael Fridmann-Sirkis, Ms. Shelly Hamer-Rogotner, Mr. Jossef Jacobovitch, Ms. Hedva Hamawi, Dr. Irina Shin, Dr. Moshe Goldsmith, Dr. Eliane Hadas Yardeni
N/A

Course Schedule and Location

2026
Second Semester
09:00 - 16:00
26/04/2026
05/05/2026

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; 2.00 points
Chemical Sciences: Laboratory; 1.00 points

Comments

In-person lab course schedule:
Part I: Protein purification & Analysis (2026)
Sunday (26/4) @ Life Science Teaching Lab + Classroom (Science Park)
9:00 - 10:30 Introduction to Proteins & Purification (Shira)
10:30 - 13:00 Purification of proteins for MST:
Affinity (Ni column & syringe)
On-column cleavage (Ni beads + bdsumo protease)
13:00 - 13:30 Lunch
13:30 - 15:00 Run gel, desalting (desalting column & dialysis)
15:00 ? 16:00 Introduction to protein-protein interactions (Yael)
Monday 27/4 @ Life Science Teaching Lab + Classroom (Science Park)
9:00 - 10:00 Handling purified proteins (Shira)
10:00 - 13:00 disassemble dialysis, protease cleavage
Determine concentration using nanodrop & Bradford reagent
Reverse IMAC (Ni cleanup)
Concentrate protein & determine its concentration using UV280
13:00 - 13:30 Lunch
13:30 - 14:30 Introduction to MST & Biacore (Yael)
14:30 - 16:00 Discussion (students describe their projects and expectations)
Tuesday (28/4)
9:00 - 10:30 Introduction to ITC, DSC and DSF (Yael) @ Benoziyo #690 @ Levin 007
13:00 - 14:00 Lunch
14:00 - 15:00 AS precipitation & gel @ Life Science Teaching Lab (Science Park)
15:00 - 16:00 Chromatography & columns (Moshe)
Wednesday (29/4)
9:00 - 10:30 FPLC @ Benoziyo lab #118
HIC followed by AEX
SEC (protein-protein interaction)
10:30-12:00 Protein purification game (Benoziyo seminar room #390C)
12:00 - 13:00 Lunch
13:00 - 16:00 Lab (Biacore, DLS/ ITC, DSC/ MST, DSF, Protein Labeling) @ Levin 007
Thursday (30/4) @ Life Science Teaching Lab + Classroom (Science Park)
9:00 - 10:00 Protein fold & refolding of IBs (Shira)
10:00 - 11:00 Refolding IBs
11:00 - 13:30 Lab (Biacore, DLS/ ITC, DSC/ MST, DSF, Protein Labeling) @ Levin 007 13:30 - 14:30 Lunch
14:30 - 16:00 Summary of course (discussion) @ Benoziyo seminar room #290

Part II: Protein crystallization, structure analysis, 3D protein modeling and PyMol tutorial
Sunday (3.5) Protein Crystallization @ Life Science Teaching Lab (Science Park)
9:00 - 10:00 Protein crystallization & Crystallography (Orly)
10:00 - 11:30 Crystallization experiment ? Hanging drop
11:30 - 12:00 Quizizz - crystallization scoring assignment
12:00 - 13:00 Lunch
13:00 - 14:00 Bioinformatic tools for protein analysis (Orly)
14:00 - 16:00 Crystallization experiment ? optimization
16:00 ? 17:00 Kahoot - crystallization scoring assignment
Monday (4.5) Protein structure analysis & structure prediction @ Life Science Teaching Lab (Science Park)
9:00 - 10:00 Correlation between sequence, function & protein structure (Orly)
10:00 - 12:00 Crystallization experiment ? seeding, PCT & Dye
12:00 - 13:00 3D Protein structure modeling (prediction)
13:00 - 14:00 Lunch
14:00 ? 16:00 Visit the SPU crystallization lab & viewing crystallization experiment results
Freeze crystals & visit the new SPU X-ray lab (Benoziyo lab #119).
Tuesday (5.5) Protein structure visualization - PyMOL @ Benoziyo seminar room #290
9:00 - 10:00 Introduction to PyMOL
10:00 - 13:00 PyMOL hands-on session
13:00 - 14:00 Lunch
14:00 - 16:00 Exam



Prerequisites

No

Restrictions

25

Language of Instruction

English

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

100%
complete attendance required

Evaluation Type

Examination

Scheduled date 1

N/A
N/A
-
N/A

Scheduled date 2

N/A
N/A
-
N/A

Estimated Weekly Independent Workload (in hours)

4

Syllabus

 ”Protein crystallization, structure analysis, modeling and visualization by PyMOL”

Bioinformatic tools for protein analysis.

Protein crystallization – techniques, strategies and tips.

Correlation between proteins sequence-function-structure.

Protein structure prediction.

Protein visualization – PyMol

“Protein purification, characterization & protein-protein interaction analysis”

Protein purification using a variety of tags & biophysical properties

Columns & FPLC

Handling purified proteins:

Concentrating proteins

Buffer exchange

Labeling

Determining protein concentration

Protein stabilization

Analysis of protein oligomeric state by SEC

Analysis of protein-protein interaction:  ITC, MST & Biacore

Analysis of protein stability by DSF & DLS

 

Install PyMOL on your computer for Tuesday, May 3rd:

Go to   WeizmannNet

https://www.weizmann.ac.il/WeizmannNet/en

Open   IT Service Catalog

Search for PyMOL

Login using Weizmann Email

Download the PyMOL

Download files for the PyMOL session

 

Learning Outcomes

Upon completion of this course students should be able to demonstrate basic knowledge and experience in protein bioinformatics, protein purification, analysis of protein-protein interactions, crystallization of proteins and 3D structure analysis.

 

Reading List

N/A

Website

N/A