Course Identification

Title:

Protein purification, crystallisation and structural analysis

Code:

20173362

Lecturers and Teaching Assistants

Lecturers:

Dr. Shira Albeck, Dr. Orly Dym

TA's:

N/A

Course Schedule and Location

Year:

2017

Semester:

Second Semester

When / Where:

11/6-15/6, 11:00-16:00 FGS Lab, FGS, Lab

First Lecture:

11/06/2017

End date:

15/06/2017

No. of hours (laboratories):

25

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; 0.50 points

Comments

Location: FGS lab on Sunday, Monday and Thursday.
ISPC- On Tuesday and Wednesday.

Prerequisites

Applicants must shortly describe how this course will contribute to their current research. Please state whether you have previous experience in either protein purification or protein crystallisation.

Restrictions

Participants:

20

Language of Instruction

English

Registration by

Registration By:01/05/2017

Attendance and participation

Obligatory

Grade Type

Pass / Fail

Grade Breakdown (in %)

Attendance

100%

Evaluation Type

Laboratory

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

3

Syllabus

Bioinformatic analysis of proteins.
Protein purification using: affinity, ion exchange, size exclusion.
Protein analysis: removal of tags, SDS PAGE, dialysis, concentration of proteins and methods to determine protein concentration.
Protein size and protein-protein interactions: size exclusion and native gels.
Crystallization of proteins
3D structure determination and preliminary structure analysis.

Learning Outcomes

Upon successful completion of this course students should be able to:

Demonstrate basic knowledge and experience in protein bioinformatics, purification, analysis, crystallization, and 3D structure determination.

Reading List

N/A

Website

N/A