This course will offer basic experience in specific aspects of cell biology. We will use mammalian tissue culture to study different aspects of cell transformation by oncogenes. Both lectures and hands-on practice will be offered.
We will compare the effect of several drugs on cell cycle cytoskeleton in normal and transformed cells. We will study the changes in the cytoskeleton and cell morphology resulting from the cancer transformation process. We will assess the effect of several cytoskeletal-perturbing drugs on the morphology and motility behavior of the cells.
Several methodologies will be employed including methods for the assessment of cell growth, FACS, immunostaining, and phase and fluorescent microscopy.