Course Identification

Title:

Introduction to advanced techniques in electron microscopy and sample preparation for life science

Code:

20183301

Lecturers and Teaching Assistants

Lecturers:

Ms. Helena Sabanay, Dr. Elena Kartvelishvily

TA's:

N/A

Course Schedule and Location

Year:

2018

Semester:

First Semester

When / Where:

29/10-14/12, Electron Microscopy Unit seminar room, Old Wolfson building, 1st floor

First Lecture:

29/10/2017

No. of hours (laboratories):

32

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; 1.00 points
Chemical Sciences: 1.00 points
Life Sciences (Systems Biology Track): 1.00 points
Life Sciences (Molecular and Cellular Neuroscience Track): 1.00 points
Life Sciences (Brain Sciences: Systems, Computational and Cognitive Neuroscience Track): 1.00 points

Comments

Lectures 29.10.17 11:00-13:30
Lectures 05.10.17 11:.00-14:00
Lectures 12.10.17 11:00-13:30

Workshop 13.11.17 11:00-17:30
Workshop 14.11.17 16:00-17:00
Workshop 15.11.17 9:00- 16:00

Workshop 19.11.17 11:00-17:00
Workshop 20.11.17 11:00-17:00
Workshop 21.11.17 11:00-17:00
Workshop 22.11.17 11:00-17:00

Lectures 0.3.12.17 11:00-13:00
Lectures 10.12.17 11:00-13:00
Lectures 14.12.17 11:00-13:00
Lectures 24.12.2017 11.00-14.00

Prerequisites

No

Restrictions

Participants:

16

Language of Instruction

English

Registration by

Registration By:23/10/2017

Attendance and participation

Obligatory

Grade Type

Pass / Fail

Grade Breakdown (in %)

Attendance

30%

Seminar:

70%

Presentation

Evaluation Type

Laboratory

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

1

Syllabus

This course provides broad coverage and practical knowledge of the latest techniques for microscopy in Life Sciences. We will cover Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), , and Super-Resolution Light Microscopy. Both the theory and practice will be covered over the course of the semester by lectures and lab workshop.

Learning Outcomes

Upon successful completion of the course students will be able to demonstrate:

Basic knowledge of the design and operation of TEM, SEM,and Super-resolution light microscope.
Basic knowledge of the specimen preparation steps for TEM, SEM, and Super-resolution light microscope including techniques, like Tokuyasu and immunolabeling. Each student will prepare his own specimens.
Basic knowledge of imaging using TEM, SEM, and Super-resolution light microscope.
Basic knowledge of cryo sample preparation and operation of cryo fixed specimens.
Ability to convert raw data into publishable format using imaging software.
Understanding the advantages and disadvantages of each imaging technique.

Reading List

N/A

Website

N/A