Course Identification

Introduction to Flow and Imaging Flow Cytometry
20203172

Lecturers and Teaching Assistants

Dr. Ziv Porat, Dr. Ekaterina Kopitman, Dr. Tomer Meir Salame, Dr. Efrat Hagai
N/A

Course Schedule and Location

2020
Second Semester
See comments section,
04/06/2020
11/06/2020
30

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; Regular; 0.50 points
Life Sciences (Molecular and Cellular Neuroscience Track): Laboratory; Elective; Regular; 0.50 points
Life Sciences (Brain Sciences: Systems, Computational and Cognitive Neuroscience Track): Laboratory; Elective; Regular; 0.50 points
Life Sciences (Computational and Systems Biology Track): Laboratory; Elective; Regular; 0.50 points

Comments

Revised course schedule:
1. On line ZOOM lectures:
4/6/2020 - 13:30-15:00
7-11/6/2020 - 9:00 -10:45

2. Practical lab sessions ? Held in the Flow Cytometry unit, Ulman building, rooms 3,8,9

3 daily sessions:

7-10/6/2020 11:00-12:30, 12:45-14:15, 14:30-16:00

You will receive detailed instructions (printed+Online) for each experiment, so each student can work independently.

3. Interactive on-line ZOOM demonstration of Imaging Flow Cytometry

11/6/2020 11:00-13:00



Prerequisites

No

Restrictions

24

Language of Instruction

English

Attendance and participation

Obligatory

Grade Type

Pass / Fail

Grade Breakdown (in %)

100%

Evaluation Type

Laboratory

Scheduled date 1

N/A
N/A
-
N/A

Estimated Weekly Independent Workload (in hours)

N/A

Syllabus

  1. Principles of operation of Flow and Image Cytometers: the flow systems, sample excitation and illumination, sample detectors.
  2. Applications of Flow and Image Cytometry: measurement of multiple color staining, DNA measurements, Nuclear - cytoplasm translocations.
  3. Cell Sorting and analysis of yield and purity.
  4. Preparation of samples.
  5. Data analysis.

Learning Outcomes

Upon successful completion of this course students should be able to:

  1. Discuss the principles and some applications of Flow and Imaging Flow Cytometry.
  2. Understand the principles of running samples on Flow and Imaging Flow Cytometers.
  3. Analyze samples with multi-color staining.
  4. Understand the principles of sorting multi-color cell populations to test tubes.
  5. Perform cell cycle analysis.

Reading List

  1. Shapiro H. M - Practical Flow Cytometry.
  2. Givan A. L - Flow Cytometry first principles.
  3. Ormerod M. G. - Flow Cytometry

Website

N/A