Low molecular weight biological molecules or so called ‘metabolites’ are vital for viability and fitness of all living creatures. Detection, identification and quantification of hundreds of thousands of metabolites possessing endless structures is a great challenge, requiring ample expertise in analytical chemistry. Yet, proficiency in such methodologies is critically needed in many labs, both biology and chemistry oriented ones. This course will offer a unique opportunity for scientists to gain fundamental knowledge in using analytical chemistry for the interrogation of metabolites in any given organism.
Chromatography and mass spectrometry are the core technologies that provide the capability to measure metabolites. The course will provide both theoretical background as well as practical experience with respect to analyzing small molecules in complex extracts. Course attendees will get acquainted with cutting edge liquid and gas chromatography coupled to mass spectrometry technologies (i.e. LC-MS & and GC-MS). The practical part will be provided in small groups. It will include hands-on activity (see Important Comment) that will cover sample preparation, analytical runs on advanced LC-MS and GC-MS systems and raw data analysis as well as downstream data processing.
Important Comment: Due to the current circumstances, the course, including theoretical and practical part will be carried out online via ‘ZOOM’. You will monitor sample preparation either by short video, or by on-line power point presentation. Other parts of the practical session will be given as an interactive ‘ZOOM’ meeting in which you will be actively involved in discussions about instrumentation, analytical runs and data analysis.
Theoretical part:
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Date
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Time
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Lecturer
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Topic
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21/6
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13:15 - 15:00
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Igal Bar Ilan
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Introduction to liquid chromatography
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21/6
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15:10 - 17:00
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Yishai Levin
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Introduction to mass spectrometry
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|
24/6
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13:15 - 14:00
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Asaph Aharoni
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Introduction to "Analysis of small biomolecules"
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24/6
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14:10 - 16:00
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Ilana Rogachev
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Non-targeted LCMS analysis
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25/6
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13:15 - 15:00
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Sergey Malitsky
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Lipidomics
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25/6
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15:10 - 17:00
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Alexander Brandis
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Targeted LCMS analysis
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28/6
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13:15 - 15:00
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Igal Bar Ilan
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GCMS analysis
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28/6
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15:10 - 17:00
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Igal Bar Ilan
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GCMS analysis
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Location of all the lectures - via ‘ZOOM’ (or FGS room A in case the current situation with Corona virus will be better), All course participants will receive a link with the invitation to join a meeting ‘ZOOM’. You will be able to log to the on-line sessions through that same link weekly.
Practical part:
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Date
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Time
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Mentors
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29/6 - 02/7
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13:00 - 17:00
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Igal (GCMS), Ilana, Sergey, Alexander
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Location of practical part – via ‘ZOOM’ (or will be announced later in case it will be permitted by FGS)