Course Identification

Magnetic Resonance: A Primer
20212251

Lecturers and Teaching Assistants

Prof. Lucio Frydman
Dr. Ricardo Pinto Enes Martinho

Course Schedule and Location

2021
First Semester
Tuesday, 14:15 - 15:00
Thursday, 14:15 - 15:00

Tutorials
Tuesday, 15:15 - 16:00,
Thursday, 15:15 - 16:00,
27/10/2020

Field of Study, Course Type and Credit Points

Chemical Sciences: Lecture; Elective; Core; 3.00 points
Life Sciences: Lecture; Elective; Regular; 3.00 points
Life Sciences (Brain Sciences: Systems, Computational and Cognitive Neuroscience Track): Lecture; Elective; Regular; 3.00 points

Comments

All courses in the first semester will be held online via zoom.

Office hours: Tuesdays, 16:15-17:00

Prerequisites

No

Restrictions

30

Language of Instruction

English

Attendance and participation

Expected and Recommended

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

33%
67%

Evaluation Type

Examination

Scheduled date 1

28/02/2021
N/A
1300-1700
N/A

Scheduled date 2

15/03/2021
N/A
1300-1700
N/A

Estimated Weekly Independent Workload (in hours)

3

Syllabus

   
0.
PERSPECTIVE

Where Magnetic Resonance comes from. Where it stands in Physics, Chemistry, Biology, Medicine.  Magnetic Resonance at the Weizmann Institute


I. CLASSICAL DESCRIPTION OF NMR

Motion of Charged Particles. Magnetic Moments and Magnetic Fields. A Glimpse into the Quantum Mechanics of NMR.  Nuclear Magnetizations. Classical Motion of the Spins.  RF Irradiation of the Spins: Rotating Frame. Problems

Primary Bibliography: Keeler, paragraphs 4.1-4.4. Claridge Ch. 2


II. PRINCIPLES OF PULSED NMR

The Pulsed NMR Experiment: Square Pulses. Quadrature Detection in the Rotating Frame.  The Trigonometrics of NMR: Fourier Analysis, Discrete Sampling, and Fast Fourier Transform.  Effects of Relaxation and the Bloch Equations.  Measuring Relaxation: Pulse Sequences.  NMR Line Shapes and the Phase of Spectral Peaks. Broadband Irradiation, Selective Irradiation, Composite Pulses.  Artifacts and Phase Cycling.  Signal?to?Noise in Pulsed NMR. Resolution Considerations: Magnets, Shims, Spinning, Locking.  Review of Electronics.  NMR Probes.  Down-conversion and digitization.  Block Diagram of a Basic NMR Spectrometer.  Problems.

Primary Bibliography: Derome, chapters 2 to 4. Fukushima, paragraphs II-A, II-B, V-C. Keeler paragraphs 4.1-4.8, 4.10-4.11. Ch. 5. Ch. 13. Claridge Ch. 3
 

III. MAGNETIC RESONANCE INTERACTIONS

The Chemical Shift and Spin?Spin (J) Interactions: Typical Couplings in Organic and Biological Spin-1/2 Systems.  The NMR Signal of Several Coupled Spins.  Equivalence in Liquid Phase NMR.  NMR Interactions in Solids vs Liquids: The Shift, Dipolar, and Quadrupolar Anisotropies.  Solid State NMR Line Shapes.  Averaging of Anisotropic Couplings in Solution NMR.  Averaging Techniques in Solids.  MR Interactions in the Presence of Unpaired Electrons: Electron Paramagnetic Resonance.  Paramagnetic NMR.  Man-made Interactions: Gradients and Magnetic Resonance Imaging.  Problems

Primary Bibliography: Claridge Ch. 2.


IV. MULTIPLE RESONANCE: DECOUPLING, EDITING, AND TRANSFERS

The Full Effects of J?Couplings: In?Phase and Anti?Phase Magnetizations.  Double Resonance_part 1: π?Pulses and Decoupling.  Composite Pulse Decoupling.  APT Spectral Editing. The Nuclear Overhauser Effect (NOE).  Double Resonance_part 2: π/2-Pulses and Coherence Transfers.  Heteronuclear INEPT Experiments.  Inept Spectral Editing and DEPT Experiments.  Problems

Primary Bibliography:  Derome, chapter 6. Keeler Ch4.9, 4.10, Ch 7. Claridge Ch. 4


V. MULTIDIMENSIONAL NMR

Principles and Plots.  Correlations via J?Couplings: Heteronuclear Correlation and Homonuclear  COSY NMR Experiments.  Purely?Absorptive Lineshapes in 2D NMR.  Digital Resolution, Selectivity and Noise in 2D NMR.  Cross Peaks Among Indirectly Coupled Spins: Relayed COSY and TOCSY NMR Experiments. Inverse Spectroscopy.  Experiments Involving Transfers of Magnetizations: 2D Exchange and 2D NOESY NMR.   Magnetic Resonance Images as Multidimensional NMR Spectra. Problems. 

Primary Bibliography: Derome, chapters 8 & 9.  Keeler Ch. 8. Claridge Chs. 5 & 6

Learning Outcomes

At the conclusion of these lectures, students should leave with an idea of what magnetic resonance is,  what its main uses are in chemistry, biophysics and imaging, and how it manages to deliver this information

Reading List

Texts:

"Modern NMR Techniques for Chemistry Research"; Derome (Pergamon, 1987). 3 physical copies available; also available online:  

https://www-sciencedirect-com.ezproxy.weizmann.ac.il/bookseries/tetrahedron-organic-chemistry-series/vol/6/suppl/C

"Understanding NMR Spectroscopy"; Keeler (Wiley, 2005). – only 1 available copy in the library, ebook link:  Accessible via the Library catalog or directly at https://weizmann.overdrive.com/media/666743
Since the book is available in electronic format only on this (Overdrive) platform, access to it is limited to 1 concurrent user and the book can only be "borrowed" for up to 21 days.

 

"Experimental Pulsed NMR: A Nuts and Bolts Approach"; Fukushima & Roeder (Addison, 1981). – 1 book available at the library

Accessible via the Library catalog or directly at 
https://www-taylorfrancis-com.ezproxy.weizmann.ac.il/books/9780429493867
We have multi-user access via the publisher


"Spin Dynamics: Basic of NMR”; M. Levitt (Wiley, 2008). – there should be 4 copies in the library accessible via the Library catalog or directly at https://weizmann.overdrive.com/media/220229
Since the book is available in electronic format only on this (Overdrive) platform, access to it is limited to 1 concurrent user and the book can only be "borrowed" for up to 21 days.

 

"High Resolution NMR Techniques in Organic Chemistry”; T.D.W. Claridge (Elsevier, 2009). A good starting book; we have it in the library as ebook and hard copy: The 3rd, 2016 year edition,  in electronic format only. It is accessible via the library catalog or directly at:
https://www-sciencedirect-com.ezproxy.weizmann.ac.il/book/9780080999869/high-resolution-nmr-techniques-in-organic-chemistry

The 2nd, 2009 year edition is also available in electronic format:
https://www-sciencedirect-com.ezproxy.weizmann.ac.il/bookseries/tetrahedron-organic-chemistry-series/vol/27/suppl/C

 

Online material:

  1. https://www.weizmann.ac.il/ISMAR/books-nmr-spectroscopy
  2. http://www-keeler.ch.cam.ac.uk/lectures/
  3. http://spindynamics.org/group/?page_id=18
  4. https://www.youtube.com/watch?v=s1am1RgrmDw&list=PL471uBfQUs9qcODBkQGJTZkcblIKrjKry

  

Website

N/A