Lecturers and Teaching Assistants
Prof. Rotem Sorek, Prof. Eran Hornstein, Prof. Igor Ulitsky
Course Schedule and Location
Tuesday, 11:15 - 13:00, Wolfson Auditorium
26/10/2021
Field of Study, Course Type and Credit Points
Life Sciences: Lecture; Elective; Regular; 2.50 points
Chemical Sciences: Lecture; Elective; Regular; 2.50 points
Life Sciences (Molecular and Cellular Neuroscience Track): Lecture; Elective; Regular; 2.50 points
Life Sciences (Brain Sciences: Systems, Computational and Cognitive Neuroscience Track): Lecture; Elective; Regular; 2.50 points
Life Sciences (Computational and Systems Biology Track): Lecture; Elective; Regular; 2.50 points
Mathematics and Computer Science (Systems Biology / Bioinformatics): Lecture; Elective; Regular; 2.50 points
Comments
The first 4 lectures (Oct 26, Nov 2 and Nov 9 Nov 16) will be given over Zoom. The rest of the lectures (starting Nov 23th) will be given as usual in the lecture hall.
Attendance and participation
Scheduled date 1
31/01/2022
Scheduled date 2
10/03/2022
Estimated Weekly Independent Workload (in hours)
Syllabus
RNA emerges in recent years as a dominant mechanism for regulation of biological processes at all levels. The aim of this course is to explore advanced topics in RNA-based regulation. The course will discuss mechanisms such as microRNA, alternative splicing, RNA editing and nonsense mediated RNA decay. A strong focus will be put on microRNA biochemistry, target selection and developmental impact. We will also discuss small RNA based regulation in prokaryotes including riboswitches, sRNA, and CRISPR.
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Rotem - alternative splicing
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Rotem - NMD and editing
Rotem - intro to lincRNAs
Rotem - bacterial ncRNAs
Rotem - CRISPR
Igor - lncRNAs
Igor - lncRNAs
Igor - other ncRNAs
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28-Dec
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Eran - miRNA intro
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4-Jan
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Eran - miRNA biogenesis
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11-Jan
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Eran - miRNA: development & disease (muscle, stem cells, cancer)
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18-Jan
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Eran - miRNA mechanism of action, circRNAs, sponges, ceRNA
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25-Jan
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Eran - RNA cell biology in biomolecular condensates
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Exercises:
- Bioinformatic discovery of miRNA genes
- How to Scan and Pick Targets
- A case study of miRNA in development
- A case study of miRNA function in human disease
- Alternative splicing analysis
- Computational approaches to characterization of non-coding RNA in bacteria
Learning Outcomes
Upon successful completion of this course students should be able to:
- Understand the multiple layers of RNA regulation including microRNAs, alternative splicing, riboswitches etc.
- Demonstrate proficiency in the recent literature in the filed.
- Incorporate RNA studies into his/her own research.