Course Identification

Methods in Neuroscience- Practical
20223152

Lecturers and Teaching Assistants

Dr. Takashi Kawashima, Prof. Ofer Yizhar, Prof. Menachem Segal, Dr. Ivo Spiegel, Prof. Ilan Lampl, Prof. Yaniv Ziv, Dr. Rita Schmidt, Prof. Michal Rivlin, Dr. Edna Furman-Haran, Dr. Asaf Benjamin, Dr. Yonatan Katz, Ms. Katayun Cohen Kashi
Daniel Deitch, Eyal Bitton, Inbal Shainer, Emmanouil Tsivourakis, Dr. Ori Roethler, Dr. Inbar Saraf-Sinik, Ravid Haruvi, Asaf Benjamin, Serena Riccitelli, Dr. Rita Schmidt, Dr. Katayun Cohen-Kashi, Alina Heukamp

Course Schedule and Location

2022
Second Semester
18-22 September 2022.,
18/09/2022
22/09/2022

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; Regular; 1.00 points
Life Sciences (Molecular and Cellular Neuroscience Track): Laboratory; 1.00 points
Life Sciences (Brain Sciences: Systems, Computational and Cognitive Neuroscience Track): Laboratory; 1.00 points
Life Sciences (Computational and Systems Biology Track): Laboratory; 1.00 points

Comments

This practical part of "Methods in Neuroscience" is obligatory for MSc students in Life Sciences - Brain Sciences, and Molecular, and is elective for all other Life Sciences Students. It will be provided after the exam period in the 2nd semester.

Specific dates for the practical course will be announced during the second semester.

The number of students in the course is not limited.

Prerequisites

"Introduction to Neuroscience: Cellular and Synaptic Physiology", or equivalent Introductory course in Neuroscience.

Restrictions

25

Language of Instruction

English

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

50%
50%

Evaluation Type

Final assignment

Scheduled date 1

N/A
N/A
-
N/A

Estimated Weekly Independent Workload (in hours)

10

Syllabus

The lab course will take place in early September 2022. This part will include extensive hands-on experiments in several labs, followed by data analysis and submission of written reports. The main emphasis will be given on techniques of neurophysiology. Techniques covered in the lab course will include, for example:

  1. Extracellular and intracellular recordings in vivo.
  2. Patch clamp recording in cultured neurons.
  3. Imaging of calcium dynamics in individual cells.
  4. Optogenetics
  5. In vivo calcium imaging in freely moving mice.
  6. MRI imaging of inanimate samples and human volunteers.

The number of participants is not limited, and each student is required to rotate in 4 labs. 

Learning Outcomes

Upon successful completion of this course students should be be able to:

  1. Demonstrate knowledge in a wide range of neuroscience methods.
  2. Read and understand research papers that utilize modern molecular approaches, neurophysiology and imaging techniques.
  3. Conduct MRI, electrophysiology and optical imaging experiments, collect and analyze neural data.
  4. Demonstrate comprehension of the rules that govern the activity of central neurons.

Reading List

  • The Axon Guide (3rd edition) - available online from various sources.
  • Imaging in Neuroscience - a Laboratory Manual (Cold Spring Harbor Press)

Website

N/A