Course Identification

Analysis of proteins: Chromatography and mass spectrometry
20223212

Lecturers and Teaching Assistants

Dr. Yishai Levin, Prof. Michal Sharon, Dr. Gili Ben-Nisan
N/A

Course Schedule and Location

2022
Second Semester
13-15 June 2022 theoretical. 26-30 June 2022 practical.,
13/06/2022
30/06/2022
28

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; Regular; 1.00 points
Chemical Sciences: Laboratory; Elective; Regular; 1.00 points
Life Sciences (Molecular and Cellular Neuroscience Track): Laboratory; Elective; Regular; 1.00 points
Life Sciences (Computational and Systems Biology Track): Laboratory; Elective; Regular; 1.00 points

Comments

13.06.22 13:00-15:00 mass spec basics-
14.06.22 13:00-17:00 proteomics basics
23.06.22 09:00-11:00 Native MS theoretical (changed from the 15th due to unexpected circumstances).
26.06.22-29.6.21 11:00-17:00 practical part in groups (Gili and Yishai)
30.06.22 09:00-11:00 exam (Gili and Yishai)
Venue: Benoziyo Auditorium, except for 26/6/22


Prerequisites

No

Restrictions

40

Language of Instruction

English

Registration by

08/05/2022

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

50%
50%

Evaluation Type

Seminar

Scheduled date 1

N/A
N/A
-
N/A

Estimated Weekly Independent Workload (in hours)

1

Syllabus

Analysis of proteins provides unparalleled insight into the function of many biological systems. Chromatography and mass spectrometry are the core technologies that provide the capability to measure proteins in their native or identify and quantify thousands of proteins in a single experiment (termed bottom up proteomics).  

The course will provide theoretical background of discovery proteomics and native protein analysis, including how raw data of typical experiments are produced, what information it provides, how to process the data and interpretation.

The course will also include live demonstrations at the de Botton Institute for Protein Profiling and at Prof. Michal Sharon’s laboratories of how to run samples on the instruments and process data.

Learning Outcomes

Upon successful completion of this course students should be able to:

  1. Demonstrate understanding of the basics of  chromatography and mass-spectrometry.
  2. Demonstrate understanding of the basics of mass spectrometry including mass spectrometry for characterizing the structural properties of protein complexes.
  3. Prepare samples for the different methods of analysis, appreciate data collection and analysis.
  4. Discuss the various applications, limitations and restrictions of the various methods.

Reading List

N/A

Website

N/A