Course Identification

Analysis and sorting by flow and image cytometry
20233191

Lecturers and Teaching Assistants

Dr. Ziv Porat, Dr. Tomer Meir Salame, Dr. Ekaterina Kopitman, Dr. Efrat Hagai
N/A

Course Schedule and Location

2023
First Semester
Introductory lecture at 10/11,
10/11/2022
17/11/2022
24

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; Regular; 1.00 points

Comments

13-17/11/2022, with an introductory lecture at 10/11/2022.

Prerequisites

No

Restrictions

24

Language of Instruction

English

Registration by

11/10/2022

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

60%
40%

Evaluation Type

Laboratory

Scheduled date 1

N/A
N/A
-
N/A

Estimated Weekly Independent Workload (in hours)

3

Syllabus

  1. Principles of operation of Flow and Image Cytometers: the flow systems, sample excitation and illumination, sample detectors.
  2. Applications of Flow and Image Cytometry: measurement of multiple color staining, DNA measurements, Nuclear - cytoplasm translocations.
  3. Cell Sorting and analysis of yield and purity.
  4. Preparation of samples.
  5. Data analysis.

Learning Outcomes

Upon successful completion of this course students should be able to:

  1. Discuss the principles and some applications of Flow and Image Cytometry.
  2. Run samples on Flow and Image Cytometers.
  3. Analyze samples with multi-color staining.
  4. Sort multi-color cell populations to test tubes.
  5. Perform cell cycle analysis.

Reading List

  1. Shapiro H. M - Practical Flow Cytometry.
  2. Givan A. L - Flow Cytometry first principles.
  3. Ormerod M. G. - Flow Cytometry

Website

N/A