Course Identification

Title:

Advanced light microscopy

Code:

20243252

Lecturers and Teaching Assistants

Lecturers:

Dr. Yoseph Addadi, Dr. Tali Dadosh, Dr. Reinat Nevo, Ms. Ofra Golani, Dr. Inna Goliand, Dr. Eyal Schejter, Dr. Melanie Horev, Ms. Tatiana Smirnova

TA's:

N/A

Course Schedule and Location

Year:

2024

Semester:

Second Semester

When / Where:

Tuesday, 11:00 - 13:00, Wolfson Auditorium

First Lecture:

09/04/2024

End date:

09/07/2024

No. of hours (laboratories):

18

Field of Study, Course Type and Credit Points

Life Sciences: Laboratory; Elective; Regular; 2.00 points
Life Sciences (Molecular and Cellular Neuroscience Track): Laboratory; Elective; Regular; 2.00 points
Life Sciences (Brain Sciences: Systems, Computational and Cognitive Neuroscience Track): Laboratory; Elective; Regular; 2.00 points
Life Sciences (Computational and Systems Biology Track): Laboratory; Elective; Regular; 2.00 points
Life Sciences (ExCLS Track): Laboratory; Elective; Regular; 2.00 points

Comments

The course won't be held on May 28. An additional session will be held instead om May 30 in FGS room B, 11:00-13:00.
June 25th and July 2nd will be held in FGS Room A.

In-person course

Prerequisites

No

Restrictions

Participants:

28

Language of Instruction

English

Registration by

Registration By:08/04/2024

Attendance and participation

Obligatory

Grade Type

Numerical (out of 100)

Grade Breakdown (in %)

Attendance

25%

Seminar:

50%

Other :

25%

Evaluation Type

Seminar

Scheduled date 1

Date / due date

N/A

Location

N/A

Time

-

Remarks

N/A

Estimated Weekly Independent Workload (in hours)

2

Syllabus

This course is designed to provide students with a comprehensive understanding of advanced light microscopy techniques and their practical applications in various scientific fields. The course includes both theoretical lectures and hands-on experience in Weizmann microscopy cores/ departmental cores.

Part 1: Lectures (11 weeks, 2h lecture)

	Date (Tuesday, 11:00 - 13:00)	Lecture name	Given by
1	09.04.2024	Microscopy basics	Sefi Addadi
2	16.04.2024	Fluorescence Microscopy	Tali Dadosh
3	30.04.2024	Fluorophores and Fluorescent Proteins	Inna Goliand
4	07.05.2024	Camera and digital image	Tatiana Smirnova
5	21.05.2024	Optical Sectioning and Confocal Microscopy	Reinat Nevo
6	~~28.05.2024~~	Specimen Preparation	Inna Goliand
6	30.05.2024	Specimen Preparation	Inna Goliand
7	04.06.2024	Image analysis considerations for imaging	Ofra Golani
8	18.06.2024	Advanced imaging methods 1	Reinat Nevo
9	25.06.2024	Advanced imaging methods 2	Sefi Addadi
10	02.07.2024	Advanced imaging methods 3	Tatiana Smirnova + Tali Dadosh
11	09.07.2024	Choosing a Microscope Platform and Core Imaging Facilities	Sefi Addadi

Part 2: Hands-On Training (4 partial days)

Hands-on dates will be coordinated during the course,

Day 1 will be carried out at the mid of the course.

Day 2 and 3 will be sometime in June/July dates will be determined by pre-registration

Day 1 (4H): Hands-on sessions in the microscopy core facilities

Demo of basic microscopy concept
Image acquisition and optimization
Image visualization

Day 2 (5H): Hands –on short project on a specific microscope (Bring your samples!)

Day 3 (4H): Advanced methods demo

Light sheet microscopy
Two-photon microscopy
STED
STORM
FRAP-FRET
FLIM
TIRF
Multiplexing

Day 4 (3H): Discussion and home assignment presentation.

Learning Outcomes

Upon successful completion of the course, students will be able to:

Understand the principles and operation of advanced light microscopes.
Select appropriate sample preparation techniques based on the specific research or imaging goals.
Understand the advantages and limitations of various imaging modes.
Plan and design experiments that incorporate advanced light microscopy as a central technique.
Understand the importance of control experiments and appropriate controls in microscopy research.
Understanding basic concepts of quantification of images.
Apply critical thinking and problem-solving skills to address challenges in microscopy and related research projects.

Reading List

iBiology Microscopy Short Course

https://www.youtube.com/watch?v=4c5ILWQmqRY&list=PLQFc-Dxlf4pSRaEk8Xi9BzS0r8-LYmwRQ&ab_channel=iBiologyTechniques

A beginner’s guide to rigor and reproducibility in fluorescence imaging experiments

https://www.molbiolcell.org/doi/full/10.1091/mbc.E17-05-0276

Community-developed checklists for publishing images and image analyses

https://pubmed.ncbi.nlm.nih.gov/37710020/

Microcourses – Jennifer Waters

https://www.youtube.com/microcourses

Website

N/A